Gram's method


Gram's method
a staining technique, using a sequence of crystal violet, iodine and safranine, used to differentiate bacteria into two large groups of species
See Also: Gram-positive, Gram-negative

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  • Gram's method — [gramz] n. [after Hans C. J. Gram (1853 1938), Dan physician] a method of staining bacteria for the purpose of classification, involving treatment with crystal violet, an iodine solution, and alcohol: the violet stain is retained by Gram positive …   English World dictionary

  • Gram's method — n. Biol. a method of differentiating bacteria by staining with a dye, then attempting to remove the dye with a solvent, for purposes of identification. Phrases and idioms: Gram positive (or negative) (of bacteria) that do (or do not) retain the… …   Useful english dictionary

  • Gram's method — Gram s′ meth′od [[t]græmz[/t]] n. lab (sometimes l.c.) a method of characterizing bacteria that involves staining a slide of fixed specimens with gentian violet, washing with alcohol, and applying a counterstain • Etymology: after Hans C. J. Gram …   From formal English to slang

  • Gram's method — /gramz/, (sometimes l.c.) a method of staining and distinguishing bacteria, in which a fixed bacterial smear is stained with crystal violet, treated with Gram s solution, decolorized with alcohol, counterstained with safranine, and washed with… …   Universalium

  • Gram's method — /ˈgræmz mɛθəd/ (say gramz methuhd) noun a method of bacterial staining in which the film is first stained with crystal violet and then with Gram s iodine solution. It permits the classification of bacteria, Gram positive species keeping the… …   Australian English dictionary

  • Gram's method — noun see Gram s stain …   New Collegiate Dictionary

  • Gram staining — (or Gram s method) is an empirical method of differentiating bacterial species into two large groups (Gram positive and Gram negative) based on the chemical and physical properties of their cell walls.cite book | last = Bergey | first = David H.… …   Wikipedia

  • Gram-negative — bacteria lose the crystal violet stain (and take the color of the red counterstain) in Gram s method of staining. This is characteristic of bacteria that have a cell wall composed of a thin layer of a particular substance (called peptidoglycan).… …   Medical dictionary

  • Gram-positive — bacteria retain the color of the crystal violet stain in the Gram stain. This is characteristic of bacteria that have a cell wall composed of a thick layer of a particular substance (called peptidologlycan). The Gram positive bacteria include… …   Medical dictionary

  • Gram's stain — or Gram stain gram n 1) a method for the differential staining of bacteria that involves fixing the bacterial cells to a slide and staining usu. with the basic dye crystal violet, treating with an iodine solution to fix the dye, washing with… …   Medical dictionary


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